7FE4
Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-14 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.505, 194.201, 112.006 |
| Unit cell angles | 90.00, 116.60, 90.00 |
Refinement procedure
| Resolution | 44.704 - 1.400 |
| Rwork | 0.157 |
| R-free | 0.17020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fe3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.526 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.755 |
| Rmeas | 0.065 | 0.820 |
| Rpim | 0.025 | 0.318 |
| Number of reflections | 451842 | 64988 |
| <I/σ(I)> | 15.6 | 2.4 |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.999 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 300 mM ammonium citrate, pH 7.0-8.0, 10 mM TCEP, 12% PEG3350 |
