7FDO

Crystal structure of transcription factor CPC in complex with EGL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	86.054, 50.358, 81.657
Unit cell angles	90.00, 97.23, 90.00

Refinement procedure

Resolution	28.701 - 1.752
R-factor	0.1993
Rwork	0.198
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rru
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (7.0.076)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.024	0.018	0.313
Rmeas	0.026	0.020	0.348
Rpim	0.010	0.008	0.147
Total number of observations	209244
Number of reflections	34121	3247	3438
<I/σ(I)>	20.9
Completeness [%]	97.5	90.2	98.9
Redundancy	6.1	6.2	5.2
CC(1/2)		0.999	0.275

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris-HCl pH 6.5, 20% PEG3350, 0.2 M ammonium sulfate