7FCW

X-ray structure of H2O-solvent lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.216, 79.216, 37.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.430 - 1.430
R-factor	0.1687
Rwork	0.167
R-free	0.18580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fcu
RMSD bond length	0.006
RMSD bond angle	1.017
Data reduction software	XDS (Mar 15,2019)
Data scaling software	XDS (Mar 15,2019)
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.820	1.450
High resolution limit [Å]	1.430	1.430
Rmerge	0.063	0.676
Rmeas	0.065	0.701
Rpim	0.017	0.182
Number of reflections	22878	1136
<I/σ(I)>	26.1
Completeness [%]	100.0	99.9
Redundancy	14.7	14.6
CC(1/2)	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	4.5	293	15-25mg/mL HEWL, 50mM sodium acetate (pH4.5), 0.1g/mL NiCl2