7FCR

Crystal structure of the N-terminal domain of mutants of Human Apolipoprotein-E (ApoE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-15
Detector	RAYONIX MX-225
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	40.642, 53.207, 85.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.820 - 1.400
R-factor	0.1717
Rwork	0.171
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gs9
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.240	45.240	1.420
High resolution limit [Å]	1.400	7.670	1.400
Rmerge	0.037	0.038	0.940
Rmeas	0.040	0.042	1.012
Rpim	0.015	0.018	0.373
Total number of observations	267298	1500	12971
Number of reflections	37333	276	1803
<I/σ(I)>	26.1	55.9	2.2
Completeness [%]	99.6	97.9	99.7
Redundancy	7.2	5.4	7.2
CC(1/2)	0.999	0.997	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.15	294	100 mM sodium cacodylate, 28% PEG 400