7FBP
FXIIa-cMCoFx1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.190, 76.830, 41.020 |
| Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
| Resolution | 41.010 - 1.990 |
| R-factor | 0.2031 |
| Rwork | 0.198 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b77 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.010 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 65611 | 2745 |
| <I/σ(I)> | 8.89 | |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.83 | |
| CC(1/2) | 0.966 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 200mM sodium acetate trihydrate pH 7.0, 20% PEG 3350 |
