7FAW

Structure of LW domain from Yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	32.014, 91.568, 118.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.179 - 2.438
R-factor	0.2054
Rwork	0.202
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model built by rosetta
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.070	2.500
High resolution limit [Å]	2.438	2.440
Rmerge	0.104	0.621
Number of reflections	24984	996
<I/σ(I)>	20.2	4.8
Completeness [%]	100.0
Redundancy	14
CC(1/2)	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris, pH 8.5, 25% PEG 3350