7FAN

Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with T35 and L-pro

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	114.522, 74.296, 75.073
Unit cell angles	90.00, 111.24, 90.00

Refinement procedure

Resolution	36.533 - 1.769
R-factor	0.1767
Rwork	0.175
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6aa0
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.15rc2_3428)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.650	1.820
High resolution limit [Å]	1.700	1.700
Number of reflections	57267	4208
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.990	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Morpheus H2