7FAM

Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with L97 and L-pro

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9804
Spacegroup name	C 1 2 1
Unit cell lengths	115.488, 74.039, 74.854
Unit cell angles	90.00, 111.24, 90.00

Refinement procedure

Resolution	52.874 - 2.420
R-factor	0.1845
Rwork	0.182
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6aa0
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.15rc2_3428)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.874	2.480
High resolution limit [Å]	2.420	2.420
Number of reflections	22556	1640
<I/σ(I)>	7.4	2.2
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.985	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Morpheus H3