7FAH
Immune complex of head region of CA09 HA and neutralizing antibody 12H5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 123.15 |
Detector technology | CCD |
Collection date | 2016-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.740, 51.122, 168.248 |
Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
Resolution | 38.606 - 3.151 |
R-factor | 0.2674 |
Rwork | 0.266 |
R-free | 0.28950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lzg 1c1e |
RMSD bond length | 0.003 |
RMSD bond angle | 0.676 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 3.150 |
Rpim | 0.077 | |
Number of reflections | 30642 | 2709 |
<I/σ(I)> | 19.2 | |
Completeness [%] | 99.8 | |
Redundancy | 11.8 | |
CC(1/2) | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M MES pH 6.5, 13% (w/v) PEG 2000 |