7FAD

Crystal structure of Xenopus GCP2-N terminal domain and Mzt2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2020-01-18
Detector	RAYONIX MX-300
Wavelength(s)	0.99
Spacegroup name	I 2 2 2
Unit cell lengths	82.878, 125.099, 204.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.957 - 3.204
R-factor	0.2094
Rwork	0.206
R-free	0.23740
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.598
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.290
High resolution limit [Å]	3.170	3.170
Rmeas	0.134
Number of reflections	18101	1608
<I/σ(I)>	25.33
Completeness [%]	98.5	89.7
Redundancy	6.5
CC(1/2)		0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Sodium formate pH 7, 12% PEG 3350