7FAC

Crystal Structure of C-terminus of the non-structural protein 2 from SARS coronavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9798
Spacegroup name	P 65
Unit cell lengths	112.834, 112.834, 91.084
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.930 - 2.710
R-factor	0.2002
Rwork	0.197
R-free	0.25700
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.131	0.072	0.658
Total number of observations	221024
Number of reflections	18044	941	897
<I/σ(I)>	8.7
Completeness [%]	99.5	98.6	98.5
Redundancy	12.2	10.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Bis-Tris (pH 6.5), 0.2M NaCl, 20% (w/v) PEG 5000 monomethy ether with IPTG