7F9X
Structure of the second OTU domain of LotA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97921 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 77.150, 94.120, 83.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.790 - 1.970 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22760 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.018 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.790 | 2.020 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.094 | 0.517 |
Rpim | 0.042 | 0.250 |
Number of reflections | 21904 | 1502 |
<I/σ(I)> | 11.1 | 2.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.9 | 6.1 |
CC(1/2) | 0.997 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 39% (w/v) PEG200, 0.1 M NaOAc pH4.5 and 100 mM NaCl |