7F9L
Crystal structure of the variable region of Plasmodium RIFIN #6 (PF3D7_1400600) in complex with LAIR1 (with T67L, N69S and A77T mutations)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-14 |
| Detector | SDMS |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.133, 94.119, 126.033 |
| Unit cell angles | 90.00, 117.94, 90.00 |
Refinement procedure
| Resolution | 41.880 - 2.700 |
| R-factor | 0.2159 |
| Rwork | 0.213 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kgr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 68571 | 68571 |
| <I/σ(I)> | 14.17 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.997 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate trihydrate pH 3.6, 40% v/v polyethylene glycol 300 |
