7F8U
Crystal structure of the cholecystokinin receptor CCKAR in complex with lintitript
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.410, 71.920, 86.050 |
Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
Resolution | 29.630 - 2.800 |
Rwork | 0.236 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zbq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.296 |
Data reduction software | XDS (VERSION Jan 26, 2018) |
Data scaling software | XSCALE (VERSION Jan 26, 2018) |
Phasing software | PHASER (1.19.2) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15523 | 1545 |
<I/σ(I)> | 5.21 | 1 |
Completeness [%] | 97.5 | |
Redundancy | 4.1 | |
CC(1/2) | 0.985 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1 M HEPES, pH7.5, 25% PEG400, 250 mM sodium tartrate ,1% 1,2 -butanediol |