7F8T
Re-refinement of the 2XRY X-ray structure of archaeal class II CPD photolyase from Methanosarcina mazei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Detector technology | CCD |
Collection date | 2008-02-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.88567 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.770, 69.770, 243.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 1.500 |
R-factor | 0.1704 |
Rwork | 0.170 |
R-free | 0.19870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xry |
RMSD bond length | 0.018 |
RMSD bond angle | 1.488 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19_4092: ???)) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.5 M LITHIUM SULFATE, 7.5% (W/V) PEG 8000 |