7F8K
Room temperature structure of bacterial copper amine oxidase determined by serial femtosecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2018-06-02 |
| Detector | MPCCD |
| Wavelength(s) | 1.771 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.750, 64.740, 93.550 |
| Unit cell angles | 90.00, 112.33, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.200 |
| R-factor | 0.1555 |
| Rwork | 0.154 |
| R-free | 0.17880 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.889 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASES |
| Refinement software | PHENIX (dev_3958) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.278 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 87284 | 8717 |
| <I/σ(I)> | 7.76 | |
| Completeness [%] | 100.0 | |
| Redundancy | 147.3 | |
| CC(1/2) | 0.986 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.4 | 289 | 1.05 M potassium-sodium tartrate in 25 mM HEPES buffer |
