7F8F

Roadblock from Odinarchaeota LCB_4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-10
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	I 21 21 21
Unit cell lengths	52.072, 59.245, 59.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 1.830
Rwork	0.193
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l7h
RMSD bond length	0.002
RMSD bond angle	0.448
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	ACORN
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.100	1.870
High resolution limit [Å]	1.830	1.830
Rmerge	0.046	0.524
Rmeas	0.050	0.566
Rpim	0.019	0.211
Number of reflections	8418	813
<I/σ(I)>	18.5	2.6
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)		0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	200 mM MgSO4 20% PEG 4000 10% glycerol