7F8A
Crystal structure of rRNA methyltransferase Erm38
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 77.663, 77.663, 101.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.260 - 1.900 |
R-factor | 0.2101 |
Rwork | 0.209 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ftf 3fuv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.260 | 48.250 | 2.030 |
High resolution limit [Å] | 1.900 | 8.830 | 1.970 |
Rmerge | 0.066 | 0.023 | 1.570 |
Rmeas | 0.067 | 0.024 | 1.603 |
Rpim | 0.013 | 0.005 | 0.315 |
Total number of observations | 6115 | 38643 | |
Number of reflections | 27116 | 317 | 1559 |
<I/σ(I)> | 30.6 | 89.2 | 2.1 |
Completeness [%] | 99.7 | 99.6 | 95.9 |
Redundancy | 25.9 | 19.3 | 24.8 |
CC(1/2) | 1.000 | 1.000 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M succinic acid pH 7, 5% glycerol |