7F88
Crystal structure of GH19 chitinase lacking the third loop structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-12-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.260, 103.647, 45.418 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 45.240 - 1.600 |
| R-factor | 0.1474 |
| Rwork | 0.146 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wh1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.427 |
| Rpim | 0.029 | 0.191 |
| Number of reflections | 70086 | 3458 |
| <I/σ(I)> | 20.7 | 2.5 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium citrate dibasic, 20 % w/v polyethylene glycol 3350, pH 5.1 |
