7F84
Crystal structure of CRISPR-associated Cas2c of Leptospira interrogans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-04-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 103.160, 103.160, 97.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.940 - 2.600 |
R-factor | 0.2117 |
Rwork | 0.209 |
R-free | 0.26960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qr0 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.869 |
Data reduction software | MOSFLM (7.3.0) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 72.940 | 72.940 | 2.720 |
High resolution limit [Å] | 2.600 | 9.010 | 2.600 |
Rmerge | 0.162 | 0.076 | 0.516 |
Rmeas | 0.167 | 0.080 | 0.532 |
Rpim | 0.040 | 0.022 | 0.129 |
Total number of observations | 140737 | 3389 | 17109 |
Number of reflections | 8390 | 246 | 1011 |
<I/σ(I)> | 12.3 | 18.6 | 4.8 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 16.8 | 13.8 | 16.9 |
CC(1/2) | 0.998 | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.3 M sodium citrate tribasic dihydrate pH 5.6, 5% 2-propanol, 20% PEG 4000, 0.2% LMA |