7F82
Structure of the bacterial cellulose synthase subunit Z in complex with cellooligosaccharides from Enterobacter sp. CJF-002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.280, 92.860, 90.500 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 42.510 - 1.300 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.18660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f81 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.030 | 0.567 |
| Rmeas | 0.033 | 0.669 |
| Number of reflections | 355775 | 57751 |
| <I/σ(I)> | 21.4 | 2.3 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 4.2 | 3.5 |
| CC(1/2) | 1.000 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-sodium tartrate, 20%(v/v) PEG3350 |
