7F81
Structure of the bacterial cellulose synthase subunit Z from Enterobacter sp. CJF-002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.267, 91.454, 89.931 |
| Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
| Resolution | 38.340 - 1.930 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qxf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.063 | 0.597 |
| Rmeas | 0.073 | 0.696 |
| Number of reflections | 107156 | 17297 |
| <I/σ(I)> | 18.26 | 2.57 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.999 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-sodium tartrate, 20%(v/v) PEG3350 |
