7F7O
APC-Asef FP assay tracer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2020-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.234, 71.619, 83.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.460 - 2.800 |
| R-factor | 0.2872 |
| Rwork | 0.285 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iz6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.460 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.649 | |
| Number of reflections | 8890 | 51835 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.990 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.0, 25% w/v PEG 3350 |
