7F7H
SARS-CoV-2 S protein RBD in complex with A8-1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.071 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.772, 75.660, 181.544 |
Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
Resolution | 36.280 - 3.190 |
R-factor | 0.2087 |
Rwork | 0.205 |
R-free | 0.27530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vsb |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.304 |
High resolution limit [Å] | 3.190 | 3.190 |
Number of reflections | 25319 | 2432 |
<I/σ(I)> | 7.065 | |
Completeness [%] | 99.2 | |
Redundancy | 3.7 | |
CC(1/2) | 0.969 | 0.585 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris, PEG 6000, 100mM NaCl |