7F77

Crystal structure of glutamate dehydrogenase 3 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	94.542, 94.542, 334.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.518 - 3.086
R-factor	0.2433
Rwork	0.241
R-free	0.29030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.080	6.670	3.100
Rmerge	0.134	0.064	1.050
Rmeas	0.142	0.069	1.110
Rpim	0.046	0.023	0.345
Number of reflections	26983	2797	2682
<I/σ(I)>	4.4
Completeness [%]	93.3	88	94.9
Redundancy	8	7.4	8.3
CC(1/2)		0.995	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	289	0.1 M Sodium Malonate, pH 3.5, and 17% PEG 3350