7F6J

Crystal structure of the PDZD8 coiled-coil domain - Rab7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2020-10-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	84.226, 84.226, 154.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.770 - 2.100
R-factor	0.2094
Rwork	0.207
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t91
RMSD bond length	0.008
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmeas	0.027	0.506
Number of reflections	36020	1773
<I/σ(I)>	53.9	7.8
Completeness [%]	99.7	100
Redundancy	11	11.3
CC(1/2)	0.997	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M Tris-HCl pH 8.0, 25% PEG 1000, 0.1 M NaCl