7F69

Crystal structure of WIPI2b in complex with ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97917
Spacegroup name	P 63
Unit cell lengths	97.944, 97.944, 65.581
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.800 - 1.500
R-factor	0.1828
Rwork	0.182
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltg
RMSD bond length	0.012
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.232	1.379
Number of reflections	57102	2848
<I/σ(I)>	9.06
Completeness [%]	98.4
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	30% w/v Pentaerythritol ethoxylate (15/4 EO/OH), 100 mM MES (pH 6.5), 50 mM Magnesium chloride