7F60

Crystal structure of auxiliary protein in complex with human nuclear protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	193.345, 87.531, 48.230
Unit cell angles	90.00, 93.68, 90.00

Refinement procedure

Resolution	48.240 - 2.850
R-factor	0.2377
Rwork	0.236
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4owr
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.270	3.020
High resolution limit [Å]	2.850	2.850
Rmerge	0.370	1.540
Number of reflections	36291	5780
<I/σ(I)>	3.47	0.8
Completeness [%]	99.0	97.4
Redundancy	3.52	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	296	0.1m Bis-tris ph5.5,45%PEG4000