7F52
Crystal Structure of IBV Nsp2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.038, 192.313, 105.749 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 49.903 - 2.559 |
| R-factor | 0.2267 |
| Rwork | 0.226 |
| R-free | 0.25960 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.559 | 2.600 |
| Rmerge | 0.010 | 0.518 |
| Number of reflections | 142273 | 2796 |
| <I/σ(I)> | 32.7 | |
| Completeness [%] | 98.5 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 4000, MES pH 6.5, proline, urea |
