7F4B

The crystal structure of the immature apo-enzyme of homoserine dehydrogenase from the hyperthermophilic archaeon Sulfurisphaera tokodaii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	57.098, 78.305, 65.551
Unit cell angles	90.00, 106.17, 90.00

Refinement procedure

Resolution	48.590 - 2.050
R-factor	0.2339
Rwork	0.231
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5avo
RMSD bond length	0.010
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.958	44.919	2.160
High resolution limit [Å]	2.050	6.480	2.050
Rmerge		0.026	0.579
Rmeas	0.079	0.031	0.676
Rpim	0.041	0.016	0.345
Total number of observations	128270	4056	19000
Number of reflections	34762	1144	5043
<I/σ(I)>	10.9	34.3	2.2
Completeness [%]	99.7	99.2	99.9
Redundancy	3.7	3.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	PEG2000, magnesium chloride, PEG400, Tris-HCl, DMSO