7F3K

Crystal structure of human YBX2 CSD in complex with m5C RNA in space group P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	75.749, 33.135, 33.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.690 - 1.760
R-factor	0.1487
Rwork	0.146
R-free	0.17570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a6j
RMSD bond length	0.006
RMSD bond angle	1.105
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.072	0.104
Number of reflections	8891	409
<I/σ(I)>	35.3
Completeness [%]	99.9
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1 M Tris pH 8.0, 3.2 M Ammonium sulfate