7F37

Crystal structure of AtaT2-AtaR2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	C 1 2 1
Unit cell lengths	105.380, 62.210, 125.050
Unit cell angles	90.00, 105.98, 90.00

Refinement procedure

Resolution	45.375 - 2.896
R-factor	0.2064
Rwork	0.202
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fvj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.120	3.000
High resolution limit [Å]	2.896	2.900
Rmerge	0.280	1.920
Number of reflections	17558	1708
<I/σ(I)>	7.5	1.4
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.993	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Potassium formate, 20% PEG 3350