7F36
TacT complexed with acetyl-glycyl-tRNAGly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.743, 121.873, 127.257 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 49.040 - 3.098 |
| R-factor | 0.2112 |
| Rwork | 0.208 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fvj |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 3.210 |
| High resolution limit [Å] | 3.098 | 3.100 |
| Rmerge | 0.237 | 1.411 |
| Number of reflections | 29107 | 2823 |
| <I/σ(I)> | 7.6 | 1.3 |
| Completeness [%] | 99.3 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.994 | 0.606 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200mM calcium acetate, 100mM sodium cacodylate, pH 6.0, 14% (v/v) PEG 8000 |
