7F2U
FmnB complexed with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.020, 66.489, 109.100 |
Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
Resolution | 27.580 - 1.984 |
Rwork | 0.204 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7esa |
RMSD bond length | 0.009 |
RMSD bond angle | 1.179 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.580 | 2.055 |
High resolution limit [Å] | 1.984 | 1.984 |
Rmerge | 0.042 | |
Number of reflections | 43550 | 3092 |
<I/σ(I)> | 11.68 | |
Completeness [%] | 91.5 | |
Redundancy | 1.5 | |
CC(1/2) | 0.993 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M sodium formate, 20% w/v Polyethylene glycol 3350 |