7F2D
Arabidopsis thaliana protease-associated domain of vacuolar-sorting receptor 1 in complex with cruciferin 1 C-terminal pentapeptide RVAAA (pH9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2018-11-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5419 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.680, 60.767, 40.594 |
Unit cell angles | 90.00, 111.62, 90.00 |
Refinement procedure
Resolution | 37.740 - 2.450 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tjx |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.740 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.037 | 0.086 |
Number of reflections | 5308 | 587 |
<I/σ(I)> | 23.1 | |
Completeness [%] | 96.8 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289.15 | 0.1M Bis-Tris, 30% w/v Polyethylene glycol monomethyl ether 2000 |