7F15

A SARS-CoV-2 neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	74.995, 74.995, 213.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.520 - 2.650
R-factor	0.2053
Rwork	0.201
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f0x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.113	0.953
Number of reflections	34387	1024
<I/σ(I)>	25.8	2.8
Completeness [%]	99.9
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3350