7F0X

A SARS-CoV-2 neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	75.369, 75.369, 186.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.030 - 2.800
R-factor	0.2261
Rwork	0.221
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7deu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	15742	2243
<I/σ(I)>	7.9
Completeness [%]	99.6
Redundancy	5.2
CC(1/2)	0.970	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2M Potassium phosphate monobasic pH4.8, 20% w/v Polyethylene glycol 3350