7F0K

Crystal structure of Kmp11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-14
Detector	MAR CCD 130 mm
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	34.899, 38.544, 142.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.320 - 3.500
R-factor	0.2594
Rwork	0.257
R-free	0.29840
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.334
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.625
High resolution limit [Å]	3.500	3.500
Number of reflections	2583	236
<I/σ(I)>	9
Completeness [%]	99.2
Redundancy	3.1
CC(1/2)	0.800	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	Ammonium Sulfate, Polyethylene glycol 4000