7F0K
Crystal structure of Kmp11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-14 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.899, 38.544, 142.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.320 - 3.500 |
| R-factor | 0.2594 |
| Rwork | 0.257 |
| R-free | 0.29840 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 2583 | 236 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.800 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | Ammonium Sulfate, Polyethylene glycol 4000 |
