7F0H
Structural and functional characterization of bovine G1P[5] rotavirus VP8* protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2019-04-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.03923 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 97.753, 102.364, 137.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.806 - 1.695 |
R-factor | 0.1678 |
Rwork | 0.167 |
R-free | 0.18750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jdb |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.695 | 1.700 |
Rmerge | 0.094 | 0.874 |
Number of reflections | 76163 | |
<I/σ(I)> | 24.004 | 2.409 |
Completeness [%] | 98.5 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.5 M ammonium sulfate, 0.1 M tri-sodium citrate dihydrate pH 5.6, 1.0 M lithium sulfate monohydrate |