7EYP
Crystal structure of Pseudomonas aeruginosa ppnP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.599, 61.599, 96.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eyj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (v1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.103 | 0.740 |
| Rmeas | 0.105 | 0.760 |
| Rpim | 0.024 | 0.171 |
| Number of reflections | 33692 | 3228 |
| <I/σ(I)> | 30.875 | 6.75 |
| Completeness [%] | 97.6 | 96.3 |
| Redundancy | 19.1 | 19.1 |
| CC(1/2) | 0.989 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |
