7EYO

Crystal structure of leech hyaluronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	43.514, 69.474, 86.739
Unit cell angles	90.00, 95.97, 90.00

Refinement procedure

Resolution	43.320 - 1.850
R-factor	0.1791
Rwork	0.177
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5la4
RMSD bond length	0.011
RMSD bond angle	1.669
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.077	0.063	0.496
Rmeas	0.084	0.068	0.540
Rpim	0.032	0.027	0.210
Total number of observations	295605
Number of reflections	43983	4515	4103
<I/σ(I)>	9.2
Completeness [%]	99.2	99.8	92.9
Redundancy	6.7	6.5	6.3
CC(1/2)		0.994	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	trisodium citrate, ED-2001