7EYM

Crystal structure of Vibrio cholerae ppnP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	55.242, 55.242, 111.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.380
R-factor	0.2064
Rwork	0.205
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eyj
RMSD bond length	0.007
RMSD bond angle	0.858
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.380	1.380
Rmerge	0.112	0.901
Rmeas	0.117	0.976
Rpim	0.032	0.364
Number of reflections	35454	6596
<I/σ(I)>	26.333	1.667
Completeness [%]	99.6	97.1
Redundancy	12.5	6.4
CC(1/2)	0.994	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium sulfate, 30% w/v Polyethylene glycol 8,000