7EY3
Double cysteine mutations in T1 lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293.15 |
| Detector technology | CCD |
| Collection date | 2020-09-17 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.174, 81.496, 100.046 |
| Unit cell angles | 90.00, 96.94, 90.00 |
Refinement procedure
| Resolution | 33.460 - 2.040 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.22150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dsn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.885 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 59606 | 107633 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.861 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.5M Sodium chloride, 0.1M Sodium citrate dihydrate pH 5.6, 2 % Ethylene imine polymer |
