7EY2
Bifunctional xylosidase/glucosidase LXYL D300N mutant with intermediate substrate xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.145, 258.225, 320.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 160.120 - 2.430 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kj0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 160.120 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.114 | 0.532 |
| Number of reflections | 227425 | 11317 |
| <I/σ(I)> | 36.3 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 20% PEG 3350, 0.1M Hepes pH 5.0, 0.2% MgCl2 |
