7EXV

GH127 beta-L-arabinofuranosidase HypBA1 covalently complexed with beta-L-arabinofuranoylamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-26
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	77.547, 77.547, 253.529
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.140 - 2.600
R-factor	0.2541
Rwork	0.251
R-free	0.30960
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3wkx
RMSD bond length	0.005
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.140	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.140	1.306
Rpim	0.045	0.412
Number of reflections	28225	3353
<I/σ(I)>	9	1.3
Completeness [%]	100.0	100
Redundancy	10.7	10.9
CC(1/2)	0.998	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.7 M sodium citrate, 0.1 M MES-NaOH (pH 6.5)