7EXO
Structure of legume lectin domain from Methanocaldococcus jannaschii in mannose bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-12-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.030, 55.030, 149.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.624 - 1.750 |
| Rwork | 0.159 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bqp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.717 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.624 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 24083 | 1299 |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.990 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium acetate, pH 4.6 and 2 M sodium formate |
