7EXH

Crystal structure of D383A mutant from Arabidopsis thaliana complexed with Galactinol.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	97.520, 103.649, 182.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.220 - 2.630
R-factor	0.1858
Rwork	0.183
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7exg
RMSD bond length	0.014
RMSD bond angle	1.783
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.220	91.220	2.780
High resolution limit [Å]	2.630	7.650	2.630
Rmerge	0.098	0.050	0.550
Rmeas	0.118	0.060	0.662
Total number of observations	326510
Number of reflections	105231	4147	17038
<I/σ(I)>	9.28	20.27	2.33
Completeness [%]	98.6	96.2	99.1
Redundancy	3.103	3.154	3.159
CC(1/2)	0.993	0.994	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.8	291.5	0.1 M Tris, PEG 2000, PGA