7EXG

Crystal structure of D383A mutant from Arabidopsis thaliana complexed with Galactose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	110
Detector technology	CCD
Collection date	2018-03-28
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	94.030, 103.736, 182.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.050
R-factor	0.1864
Rwork	0.184
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7exf
RMSD bond length	0.019
RMSD bond angle	2.007
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.075	0.053	0.554
Rmeas	0.082	0.058	0.606
Rpim	0.032	0.023	0.241
Total number of observations	714794
Number of reflections	111655	11589	10589
<I/σ(I)>	10.1
Completeness [%]	99.3	98.6	95.4
Redundancy	6.4	5.8	5.8
CC(1/2)		0.996	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.8	291.15	0.1 M Tris, PEG 2000, PGA