7EX9

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.855, 70.763, 70.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.420 - 1.750
R-factor	0.1856
Rwork	0.185
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.052	0.032	0.381
Rmeas	0.060	0.038	0.458
Rpim	0.030	0.020	0.249
Total number of observations	77608
Number of reflections	20986	2096	2056
<I/σ(I)>	15.1
Completeness [%]	97.7	90.2	98.5
Redundancy	3.7	3.4	3.2
CC(1/2)		0.998	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.1	289	PEG 3350