7EX2

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with L-742,001 (methyl ester form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	40.409, 70.804, 69.860
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	26.440 - 3.100
R-factor	0.1826
Rwork	0.179
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.104	0.059	0.252
Rmeas	0.121	0.067	0.293
Rpim	0.060	0.032	0.146
Total number of observations	22253
Number of reflections	6248	613	618
<I/σ(I)>	6.9
Completeness [%]	85.1	81.2	84.8
Redundancy	3.6	3.5	3.5
CC(1/2)		0.997	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.3	289	PEG 3350